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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/12/2010 |
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Data da última atualização: |
19/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. |
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Afiliação: |
Eliane Cristina Locali-Fabris, APTA; Valéria S. Mafra, APTA; Polyana Kelly. Martins, APTA; Marcelo Ribeiro-Alves, Fiocruz; Carolina S. Francisco, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Luciano T. Kishi, APTA; Marcelo A. Machado, APTA. |
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Título: |
Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
0058 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010 |
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Conteúdo: |
Huanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001. MenosHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, includin... Mostrar Tudo |
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Palavras-Chave: |
Huanglongbing; Plant disease. |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Candidatus Liberibacter; Citrus. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02656nam a2200277 a 4500
001 1870461
005 2011-01-19
008 2010 bl uuuu u00u1 u #d
100 1 $aLOCALI-FABRIS, E. C.
245 $aGene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus.
260 $aIn: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM.$c2010
500 $a0058 PSO Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010
520 $aHuanglongbing (HLB) is known as the most devastating disease of citrus. Two species of Candidatus Liberibacter have been reported in Brazil, Ca. L. asiaticus (CaLas) and Ca. L. americanus (CaLam). Since CaLas is prevalent worldwide, most of the studies have focused that species and very little information is available on CaLam. With the objective to investigate differences in metabolic pathways of Citrus sinensis in response to CaLam, we used a genome- wide microarray approach to identify differentially expressed genes in symptomatic plants when compared to control plants. Hybridizations were performed by Roche Nimblegen with a customized 385K chip containing 32,000 unigenes of C. sinensis. The raw data were pre-processed using the Robust Multiarray Average (RMA). Normalized data were imported to R/Bioconductor where statistical analyses were performed. A Bayesian moderated t-test was calculated and p-values were adjusted for multiple comparisons by the false discovery rate correction. A total of 634 genes with P-values ?0.05, fold change |FC| ? 2.0 and odds probability ? 0.95 were considered differentially expressed. From those, 419 were up- and 215 down-regulated in symptomatic plants. In order to identify enriched gene ontologies regulated in infected plants we applied a gene set enrichment analysis approach and found 45 different biological processes including those involved in response to jasmonic acid stimulus, signaling and metabolism, 17 molecular functions, including oxygen binding, monooxygenase activity and oxidoreductase activity, 6 cellular components, mostly related to chloroplast, enriched at a statistically significant level of 0.001.
650 $aCandidatus Liberibacter
650 $aCitrus
650 $aDoença de Planta
650 $aFruta Cítrica
653 $aHuanglongbing
653 $aPlant disease
700 1 $aMAFRA, V. S.
700 1 $aMARTINS, P. K.
700 1 $aRIBEIRO- ALVES, M.
700 1 $aFRANCISCO, C. S.
700 1 $aFREITAS ASTUA, J. de
700 1 $aKISHI, L. T.
700 1 $aMACHADO, M. A.
Download
Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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| Registros recuperados : 76 | |
| 3. |  | MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis as a model plant for functional genomic studies in C4 Crops. In: KUMAR, S.; BARONE, P.; SMITH, M. Transgenic plants: methods and protocols. Berlim, Alemanha: Springer Science+Business Media, 2019. (Methods in Molecular Biology, v. 1864). p. 49-64| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | OYA, T.; MARTINS, P. K.; CASAGRANDE, E. C.; LUGLE, S. M.; NEPOMUCENO, A. L.; NEUMAIER, N.; FARIAS, J. R. B. Agronomic, physiological and molecular characteristics of drought-tolerant Brazilian soybean cultivars. In: EMBRAPA SOYBEAN/JICA-CETAPAR/INTA-EEA/JIRCAS JOINT WORKSHOP ON SOYBEAN IMPROVEMENT, PRODUCTION AND UTILIZATION IN SOUTH AMERICA, 2001, Londrina. Abstracts and program. Londrina: Embrapa Soybean: JIRCAS, 2001. p. 17. (Embrapa Soja. Documentos, 170).| Biblioteca(s): Embrapa Soja. |
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| 5. | | RIBEIRO, A. P.; MARTINS, P. K.; DIAS, B. B. A.; KOBAYASHI, A. K.; ANDRADE, A. C.; MOLINARI, H. B. C. Overexpression of the MATE gene in Setaria viridis. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 11.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | MAFRA, V.; MARTINS, P. K.; FRANSCISCO, C. S.; RIBEIRO-ALVES, M.; ASTUA, J. de F.; MACHADO, M. A. Candidatus Liberibacter americanus induces significant reprogramming of the transcriptome of the susceptible citrus genotype. In; INTERNATIONAL RESEARCH CONFERENCE ON HUANGLONGBING , 3., 2013, Orlando, Flórida, USA. IRC HLB III. Proceedings. Orlando, Flórida, USA, 2013, (online).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis: a tool for functional gene analysis in sugarcane. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 19.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | SILVA, M. F. e; CARDINALI, M. C. B.; MARTINS, P. K.; ASTÚA, J. F.; MILORI, D. M. B. P.; MASCARENHAS, Y. P. Aplicação da espectroscopia na região do infravermelho com transformada de fourier (FTIR) no estudo da doença Greening em plantações de citros. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 24. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 12. | | SILVA, M. F. e; MILORI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; ASTÚA, J. F.; MACHADO, M. A. Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. Citrus research & technology, Cordeirópolis, v. 31, suplemento, p. 120, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas. 1CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | SILVA, M. F. e; MIROLI, D. M. B. P.; CARDINALI, M. C. B.; MASCARENHAS, Y. P.; MARTINS, P. K.; FREITAS ASTUA, J. de; MACHADO, M. A. Application of fourier transform infrared spectroscopy (FTIR) for early diagnosis of citrus greening disease. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 120_PS2
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | MARTINS, P. K.; JORDÃO, B. Q.; BRETON, M. C.; PEDROSO, J. C.; MARIN, S. R. R.; BINNECK, E.; OYA, T.; NEPOMUCENO, A. L. Avaliação dos efeitos do déficit hídrico na expressão gênica em raízes de soja [Glycine max (l.) Merrill] In: CONGRESSO NACIONAL DE GENÉTICA, 48., 2002, Águas de Lindóia. A genética na inclusão social: resumos. Águas de Lindóia: SBG, 2002. 1 CD-ROM. Resumo Área MG pdf.091.| Biblioteca(s): Embrapa Soja. |
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| 15. | | VENANCIO, A. L.; CARDINALLI, M. C. B.; SILVA, M. F.; MILORI, D. M. B. P.; FERREIRA, E. J.; MARTINS, P. K.; FREITAS-ASTÚA, J. Avaliação da precocidade do diagnóstico do Greening utilizando espectroscopia de fluorescência induzida por laser (LIFS). In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 36. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 16. | | SOUSA, C. A. F. de; DIAS, B. B. A.; MARTINS, P. K.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T. Nova abordagem para a fenotipagem de plantas: conceitos, ferramentas e perspectivas. Revista Brasileira de Geografia Física, v. 8, p. 660-672, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | Ribeiro, A. P.; Martins, P. K.; DIAS, B. B. A.; ANDRADE, A. C.; MAGALHAES, J. V. de; KOBAYASHI, A. K.; MOLINARI, H. B. C. MATE: a promising gene for aluminum tolerance in sugarcane. In: GERMPLASM AND BREEDING, 11.; MOLECULAR BIOLOGY ISSCT WORKSHOP, 8., 2015, Saint-Gilles Réunion Island. Pushing the frontiers of sugarcane improvement: abstract. [S.l]: Ercane, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | DIAS, B. B. A.; KOBAYASHI, A. K.; GOMES, A. de P. G.; RODRIGUES, D. de S.; MOLINARI, H. B. C.; FAVARO, L. C. de L.; MARTINS, P. K. Manual de Biossegurança da Embrapa Agroenergia (CQB 345/12). Brasília, DF: Embrapa Agroenergia, 2015. 35 p. ( Embrapa Agroenergia. Documentos, 19).| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | BRETON, M. C.; MARTINS, P. K.; PEDROSO, J. C.; GOMES, E. V.; RUAS, C. F.; NEUMAIER, N.; NEPOMUCENO, A. L.; OYA, T.; FARIAS, J. R. B. Identificação de genes diferencialmente expressos em soja [Glycine max (L. Merril)] expostas a dois fotoperíodos. In: CONGRESSO NACIONAL DE GENÉTICA, 47., 2001, Águas de Lindóia. A genética no século XXI: desafios. [Brasilia]: Sociedade Brasileira de Genética, 2001. 1 CD-ROM. Resumo Área Melhoramento pdf. 432.| Biblioteca(s): Embrapa Soja. |
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| 20. | | PEDROSO, J. C.; MARTINS, P. K.; BRETON, M. C.; NEPOMUCENO, A. L.; FARIAS J. R. B.; NEUMAIER, N.; SILVA, J. F. V. Identificação de genes diferencialmente expressos em cultivares de soja [Glycine max (L. MerrilL)] e tolerância a infestação de nematóide da galha (Meloidogyne javanica). In: CONGRESSO NACIONAL DE GENÉTICA, 47., 2001, Águas de Lindóia. A genética no século XXI: desafios. [Brasilia]: Sociedade Brasileira de Genética, 2001. 1 CD-ROM. Resumo Área Melhoramento pdf. 1062.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 76 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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